激光共聚焦同步双扫描(simultaneous, SIM)技术在常规扫描单元的基础上, 引入一个同步扫描单元(SIM scanner), 该技术独立控制了两个激光束, 一个用于激光光刺激, 另一个用于同步成像。本实验中, 采用激光共聚焦同步双扫描系统的405 nm和488 nm激光分别对细胞的特定部位进行刺激和同步成像, 实时检测了LC3复合物的形成, 记录并分析了乙酰化前后LC3的光动力学变化过程, 证实了LC3的脱乙酰化修饰是自噬性降解所必须的, 本实验体系为激光共聚焦双扫描技术的推广提供了一个很好的平台。SIM技术的应用, 解决了刺激过程无法成像的问题, 为漂白后荧光恢复(fluorescence recovery after photobleaching, FRAP)、漂白后荧光损失(fluorescence loss in photobleaching, FLIP)和光诱导激活等研究提供了最佳的解决方案, 可作为光刺激的一种实验模式在很多实验设计中进行延伸应用。

Three-dimensional-ordered Yb/Er co-doped Bi2Ti2O7 inverse opal, powder, and disordered reference samples are prepared and their upconversion (UC) emission properties and mechanisms are investigated. Significant suppression of UC emission is detected when the photonic band-gaps overlap with Er3+ UC green emission bands. Interestingly, green and red UC emissions follow a two-photon process in the powder sample but a three-photon one in the inverse opal.